Anti - Anti - Idiotype Effect on Antibody Response
نویسندگان
چکیده
It has recently been shown that an idiotypic determinant(s) (460-Id) 1 found on the 2,4-dinitrophenyl (DNP) and 2,4,6-trinitrophenyl (TNP)-binding myeloma protein, MOPC-460, is expressed on some anti-TNP antibodies produced by BALB/c mice after immunization with certain thymus-independent (TI) (1) and thymus-dependent (2) TNP antigens. In vitro studies indicate that the 460-Id-bearing component of the anti-TNP response to the TI antigen, TNP-Nocardia water-soluble mitogen (NWSM), is regulated by a 460-Id-specific suppressor T cell found in the spleen of normal BALB/c mice (1). In this communication, we report the results of experiments in which we studied the effect of anti-460-Id antibodies and ofanti-[anti-460-Id] antibodies on the response to two TI TNP antigens. Mice actively immunized with MOPC-460 or acutely pretreated with anti-460-Id antibodies made an anti-TNP response to TNP-NWSM and to TNP-levan which lacked a 460-Id + component. By contrast, mice actively immunized with purified anti-460-Id antibodies or acutely pretreated with anti-[anti460-Id] antibodies showed an increase in the 460-Id + component of their anti-TNP response. Furthermore, T cells from mice immunized to anti-460-Id antibodies failed to suppress, in vitro, the 460-Id + component of the response of normal B cells to TNPNWSM. These results suggest (a) that 460-Id-specific suppressor T cells normally regulate in vivo responses to TI TNP-antigens; (b) that anti-[anti-460-Id] antibodies can eliminate 460-Id-specific suppressor T cells; and (c) that 460-Id-specific suppressor T cells and anti-460-Id antibodies share idiotypic determinants.
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